PSC Frontier Seminar series
Title: Mechanism and control of T-DNA integration into the plant genome
Speakers: Prof.Stanton B. Gelvin & Prof. Lan-Ying Lee,Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 USA
Day/Date: March 22nd,2019(Friday)
Host: Prof.Jian-Kang Zhu
Venue: PSC auditorium
Agrobacterium-mediated transformation is the most common method to generate transgenic plants and to introduce genome engineering reagents into plants. Genome engineering generally requires, or has as an unintended consequence, chromosomal integration of these reagents. To develop transgene-free crops, T-DNA encoding these reagents must be segregated out, which can be costly and time-consuming. Segregation is not feasible for species with long generation times (e.g., trees) or which are propagated vegetatively (e.g., potato). They are attempting to develop Agrobacterium strains that can efficiently deliver but not integrate T-DNA. Their current approach is to alter VirD2, the protein covalently linked to T-DNA that participates in T-strand nuclear targeting and perhaps T-DNA integration. A mutant version of VirD2 that is preferentially deficient in T-DNA integration but supports somewhat lower transient transformation already exists (omega mutation). They are attempting to improve upon this version of the protein.
The seminar will be delivered in English.