PSC Frontier Seminar series
Title: Engineering of new CRISPR/Cas12 orthologs for genome editing and DNA detection
Speaker: Prof. Wei Li
Time: 10:00 am
Day/Date: Apr. 9th, 2019 (Tuesday)
Host: Prof. Jian-Kang Zhu
Venue: PSC auditorium
The type V CRISPR/Cas systems have unique features distinguished from the type II CRISPR/Cas9 system that has been adapted and widely used for genome editing. Currently only three type V-A CRISPR/Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage still requires improvements. Here we report our progresses in engineering of new type V CRISPR/Cas systems for genome editing and DNA detection, which include i) repurposing CRISPR/Cas12b as the third CRISPR-based genome editing tool and six novel CRISPR/Cas12a orthologs for genome editing in human and mouse cells; ii) optimizing guide RNA scaffold to increase the genome editing efficiency of these novel systems; iii) developing a CRISPR/Cas12b-based method for rapid and efficient DNA detection.
The talk will be delivered in Chinese.