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基因组学平台



平台简介 仪器介绍及原理 技术提供 管理人员及其联系方式
平台简介 Top
高通量测序技术正在改变基因组学领域和我们研究很多生物学问题的方式。基因组学平台的目标是利用最好的技术为研究者提供完整和高性价比的基因组学解决方案。平台目前配备有两台Illumina高通量测序仪:HiSeqTM 2500和MiSeqTM,每日最高数据产量可达95 Gb。我们在各种应用上都有充足的经验,例如:重亚硫酸盐测序(DNA甲基化),RNA测学,染色体免疫沉淀测序(RNA-seq),全新测序和定向重测序。我们的二代测序服务不仅面向本中心的研究人员,也面向全世界的研究者。
仪器介绍及原理 Top

Mi-seq技术指标

READ LENGTH (BP) TOTAL TIME* OUTPUT
1 × 36 ~4 hrs 540-610 Mb
2 × 25 ~5.5 hrs 750-850 Mb
2 × 100 ~16.5 hrs 3.0-3.4 Gb
2 × 150 ~24 hrs 4.5-5.1 Gb
2 × 250 ~39 hrs 7.5-8.5 Gb
2 × 300** > 48 hrs ~15 Gb

Hi-seq 2500技术指标

HIGH OUTPUT RUN MODE*
Read Length Dual Flow Cell
(HiSeq 2500 only)
Single Flow Cell
(HiSeq 1500 or 2500)
Dual Flow Cell Run Time
1 × 36 95-105 Gb 47-52 Gb 2 days
2 × 50 270-300 Gb 135-150 Gb 5.5 days
2 × 100 540-600 Gb 270-300 Gb 11 days
2 × 150 N/A N/A N/A
Reads Passing Filter Up to 3 billion single reads or 6 billion paired-end reads Up to 1.5 billion single reads or 3 billion paired-end reads  
Quality Greater than 85% of bases above Q30 at 2 × 50 bp
Greater than 80% of bases above Q30 at 2 × 100 bp

RAPID RUN MODE*
Read Length Dual Flow Cell
(HiSeq 2500 only)
Single Flow Cell
(HiSeq 1500 or 2500)
Dual Flow Cell Run Time
1 × 36 18-22 Gb 9-11 Gb 7 hr
2 × 50 50-60 Gb 25-30 Gb 16 hr
2 × 100 100-120 Gb 50-60 Gb 27 hr
2 × 150 150-180 Gb 75-90 Gb 40 hr
Reads Passing Filter Up to 600 million single reads or 1.2 billion paired-end reads Up to 300 million single reads or 600 million paired-end reads  
Quality Greater than 85% of bases above Q30 at 2 × 50 bp
Greater than 80% of bases above Q30 at 2 × 100 bp
Greater than 75% of bases above Q30 at 2 × 150 bp

*Install specifications based on Illumina PhiX control library at supported cluster densities (between 610-678 K clusters/mm2 passing filter using TruSeq v3 Kits or 700-820 clusters/mm2 passing filter using TruSeq Rapid Kits. Run times for rapid run mode correspond to on-board cluster generation (1.5 hr) and sequencing; for high output mode, run times correspond to sequencing only. Performance may vary based on sample quality, cluster density, and other experimental factors. Early HiSeq 2000 instruments will run slightly slower when upgraded to a HiSeq 2500.

技术提供 Top

sample_submission_v2.5.xlsx

管理人员及其联系方式 Top
联系人:张蘅
联系方式:psc.genomics@sibs.ac.cn