报告人：Prof. Xinguang Zhu, CAS-MPG Partner Institute for Computational Biology, SIBS, CAS
报告人：Prof. Xueping Zhou
201710.13【PSC Frontier Seminar Series】:Calcium signaling is required for ABA mediated transcriptional adaptation to abiotic stresses报告时间：2017-10-14
电话：(86) 21- 57078279
A master regulator of immunity, OsRac1, and R protein signaling
Resistance (R) proteins are crucial intracellular receptors that detect attack by insects and invasion by various pathogens including fungi, bacteria and viruses. However, the signaling molecules that mediate R protein-induced immune responses are not yet fully understood. We have previously shown that an intracellular switch, the small GTPase OsRac1, is a master regulator controlling immunity in rice (Kawano et al., 2010b, Kawano and Shimamoto, 2013). However, the mechanism by which OsRac1 receives signals from the immune receptors and becomes activated has remained unclear. Thus, we explored OsRac1-binding proteins and identified the R protein Pit, which is a immune receptor for rice blast fungus, a prominent microbial disease of rice (Kawano et al., 2010a). Through various analyses, we demonstrated that OsRac1 functions as a molecular switch, controlling ROS production and hypersensitive cell death downstream of Pit. In addition, we have found that anchoring Pit to the plasma membrane through palmitoylation, a type of lipid modification is required for Pit-induced activation of OsRac1 on the plasma membrane (Kawano et al., 2014). Our work has therefore revealed the signaling pathway of the R protein Pit through OsRac1.
In vivo monitoring of OsRac1 activity and chitin-triggered immunity
Given that the small GTPase OsRac1 is a master regulator controlling rice immunity, monitoring its activation within plant cells was believed to be the next key step in understanding plant immunity. By creating a bio-imaging sensor using the Forester resonance energy transfer (FRET) of fluorescent proteins, we were the first to successfully monitor the activation of a plant small GTPase in vivo (Kawano et al., 2010a). Using this sensor, we identified the R protein Pit as an immune receptor that activated OsRac1 on the plasma membrane (Kawano et al., 2010a), and observed that OsRac1 was activated within 3 min after sensing chitin, a cell wall component of rice blast fungus (Akamatsu et al., 2013). Furthermore, we revealed that the signal from the chitin receptor OsCERK1 was transmitted to OsRac1 through the OsRac1 activator protein OsRacGEF1. We have also reported that OsRac1 controls the expression of defense-related genes through the MAPK OsMPK6 and the transcription factors RAI1 and Rap2.6 (Kim et al., 2012, Wamaitha et al., 2012).
Homologous recombination-based gene targeting of OsRac1 to enhance rice immunity
Over expression of defense-related proteins often causes stress and results in a reduction of biomass. Thus, technological innovation has been considered essential to provide plants with improved resistance to diseases while maintaining biomass. Rice transformed with constitutively active OsRac1 (OsRac1 G19V), which was over expressed using the 35S promoter that allows constant high expression, displays improved resistance against pathogens, but a reduced yield of rice because of a reduction in fertility. Therefore, rather than utilizing conventional gene transfer methods, we applied a gene-targeting method that uses homologous recombination, developed by Dr. R. Terada of Meijo University. Using this method, we introduced a point mutation that makes the endogenous OsRac1 gene constitutively active and investigated whether disease resistance in rice could be improved, thereby avoiding agriculturally unfavorable characters (Dang et al., 2013). Because selection markers can be removed through site-specific recombination, the gene-targeting method utilized in this study is a groundbreaking, marker-free genetic engineering technique with the ability to modify targeted genes as closely as possible to natural mutations. Introduction of the constitutively active mutation into the OsRac1 gene caused an increase in the expression of transcription factors such as WRKY, and of defense-related genes including PAL1 and PBZ1. A resistance response similar to hypersensitive cell death was also observed at the site of rice blast fungus infection.