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Services |
Analytical methods |
price
(RMB) /sample |
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1. Identification of proteins (post-translational modifications) |
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Accurate mass determination of a purified protein or peptide |
High-resolution MS/ MALDI-TOF-TOF |
300 RMB |
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Sequencing of peptides |
High-resolution MS/ MALDI-TOF-TOF |
4000 RMB |
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Identification of purified proteins OR for IP samples (phosphorylation, acetylation, ubiquitination, etc) |
In-solution digestion(*)-Peptide desalting- LC MS/MS- Database searching |
1800 RMB |
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Identification of proteins in gel (phosphorylation, acetylation, ubiquitination, etc) |
In-gel digestion(*)-Peptide desalting- LC MS/MS- Database searching |
2000 RMB |
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Identification of digested peptides (phosphorylation, acetylation, ubiquitination, etc) |
Peptide desalting- LC MS/MS- Database searching |
1500 RMB |
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Identification of phosphorylation sites of IP samples (enrichment of phosphorylated peptides) |
In-solution digestion(*)-enrichment of phosphorylated peptides-
Peptide desalting- LC MS/MS- Database searching |
2500 RMB |
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Database searching |
Mascot、PD or Maxquant |
100 RMB |
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Note: * Double digestion will increase the fee |
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2. Analysis of High - throughput proteomics |
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Label-free quantification of proteome
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Protein extraction- Protein desalting- In-solution digestion(double digestions)- Peptide desalting-( Peptides fractionation- Peptide desalting)- quantification of peptides- LC MS/MS- Database searching and analysis |
4000RMB
(Fractionation will increase the fee according to machine-hour) |
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TMT quantification of proteome
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Protein extraction- Protein desalting- In-solution digestion(double digestions)- Peptide desalting and quantification - TMT labeling and desalting -(Peptides fractionation- Peptide desalting)- quantification of peptides- LC MS/MS- Database searching and analysis |
5000RMB
(Fractionation will increase the fee according to machine-hour) |
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Label-free quantification of Phosphoproteome |
Protein extraction- Protein desalting- In-solution digestion(double digestions)- Peptide desalting and quantification-enrichment of phosphorylated peptides-
Peptide desalting -(Peptides fractionation- Peptide desalting)- quantification of peptides- LC MS/MS- Database searching and analysis |
6000 RMB
(Fractionation will increase the fee according to machine-hour) |
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TMT quantification of Phosphoproteome |
Protein extraction- Protein desalting- In-solution digestion(double digestions)- Peptide desalting and quantification - TMT labeling and desalting- enrichment of phosphorylated peptides- Peptide desalting and quantification
-( Peptides fractionation- Peptide desalting)- LC MS/MS- Database searching and analysis |
7000 RMB
(Fractionation will increase the fee according to machine-hour) |
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TMT quantification of ubiquitproteome |
Protein extraction- Protein desalting- In-solution digestion(double digestions)- Peptide desalting and quantification - TMT labeling and desalting- enrichment of ubiquited peptides- Peptide desalting and quantification
-( Peptides fractionation- Peptide desalting)- LC MS/MS- Database searching and analysis |
10000 RMB (Fractionation will increase the fee according to machine-hour) |
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PRM |
First, the PRM uses the quadruple to select the precursor ion with the selection window m/z≤2; the precursor ion is fragmented, and then all product ions are detected by Orbitrap with high resolution and high accuracy. |
2500RMB/protein |
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MRM |
First, the MRM uses the quadruple (Q1) to select the precursor ion; then, the precursor ion is fragmented in the collision cell (Q2); finally, Q3 scans targeted product ions with high sensitivity. |
2500RMB/protein |
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Orbitrap Fusion,
Q Exactive HF-X |
machine-hour (no more than two hours) |
800RMB/sample |
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Q Exactive, Q Exactive plus, QTRAP 6500+ |
machine-hour (no more than two hours) |
600RMB/sample |
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Database searching |
Mascot、PD or Maxquant |
100 RMB |
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3. Metabolomics |
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3.1 GC-MS (A) |
GC-MS |
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Without any treatment (A1) |
GC-MS- Database searching |
500 RMB |
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Complex samples (A2) |
sample purification or derivatization- GC-MS- Database searching |
800 RMB |
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Complex samples (A3) |
sample purification and derivatization- GC-MS- Database searching |
1000 RMB |
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Absolut quantification (A4) |
GC-MS-data analysis |
600 RMB |
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3.2 LC-UV (B) |
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Without any treatment (B1) |
UPLC-UV |
300 RMB |
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Complex samples (B2) |
sample purification or derivatization- UPLC-UV-data analysis |
500 RMB |
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Complex samples (B3) |
sample purification and derivatization- UPLC-UV-data analysis |
700 RMB |
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Absolut quantification (B4) |
UPLC-UV-data analysis |
500 RMB |
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3.3 Plant hormone quantification (ABA/JA/SA/IAA/GA/SLs)(C) |
UPLC-MS |
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1 plant hormone |
plant hormones extraction- UPLC-MS- data analysis |
600 RMB
( If multiple hormones are measured at the same time, the cost will increase according to 200 RMB/ hormone) |
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3.4 Plant metabolites quantification (L-Glu/GABA/ O-Acetyl-Serine etal.) (D) |
UPLC-MS |
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1 plant metabolite |
plant metabolites extraction- UPLC-MS- data analysis |
600 RMB
( If multiple metabolites are measured at the same time, the cost will increase according to 200 RMB/metabolite ) |
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High Speed Centrifuge |
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20 RMB/hour |
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Super High Speed Centrifuge |
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50RMB/hour |
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Freeze-dryer |
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20 RMB/hour |
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AKTA Protein purification system |
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300 RMB/ sample |
Introduction
Plant Proteomics and Metabolomics utilize the state-of-the-art instrumentation of chromatography and mass spectrometry, and multi-dimensional omics strategies to conduct simultaneous qualification and quantification of the entire proteome and metabolome in plant cells and tissues. Large-scale analysis of protein population, abundance changes and molecular interactions allows us to understand the environmental impacts on plant physiology, and therefore to decipher cellular signaling transduction pathways. Structural characterization of organic metabolites and protein post-translational modifications could gain in-depth insights into the stress adaptation of plants in harsh environmental conditions, as well as the diversity of metabolic pathways and other cellular mechanisms which are involved in plant growth and development, regulation, molecular cross-talk, stress response and defense. As plant proteome and metabolome are highly dynamic in living systems, the integrated strategy, using both proteomic and metabolomic analyses, thus provides a global and reliable approach to investigate unknown biological pathway events related to the survival of plants, and the interaction of multiple pathways in response to environmental stimuli and stresses.
