Services |
Analytical methods |
price
(RMB) /sample |
1. Identification of proteins (post-translational modifications) |
Accurate mass determination of a purified protein or peptide |
High-resolution MS/ MALDI-TOF-TOF |
300 RMB |
Sequencing of peptides |
High-resolution MS/ MALDI-TOF-TOF |
4000 RMB |
Identification of purified proteins OR for IP samples (phosphorylation, acetylation, ubiquitination, etc) |
In-solution digestion(*)-Peptide desalting- LC MS/MS- Database searching |
1800 RMB |
Identification of proteins in gel (phosphorylation, acetylation, ubiquitination, etc) |
In-gel digestion(*)-Peptide desalting- LC MS/MS- Database searching |
2000 RMB |
Identification of digested peptides (phosphorylation, acetylation, ubiquitination, etc) |
Peptide desalting- LC MS/MS- Database searching |
1500 RMB |
Identification of phosphorylation sites of IP samples (enrichment of phosphorylated peptides) |
In-solution digestion(*)-enrichment of phosphorylated peptides-
Peptide desalting- LC MS/MS- Database searching |
2500 RMB |
Database searching |
Mascot、PD or Maxquant |
100 RMB |
Note: * Double digestion will increase the fee |
2. Analysis of High - throughput proteomics |
|
Label-free quantification of proteome
|
Protein extraction- Protein desalting- In-solution digestion(double digestions)- Peptide desalting-( Peptides fractionation- Peptide desalting)- quantification of peptides- LC MS/MS- Database searching and analysis |
4000RMB
(Fractionation will increase the fee according to machine-hour) |
TMT quantification of proteome
|
Protein extraction- Protein desalting- In-solution digestion(double digestions)- Peptide desalting and quantification - TMT labeling and desalting -(Peptides fractionation- Peptide desalting)- quantification of peptides- LC MS/MS- Database searching and analysis |
5000RMB
(Fractionation will increase the fee according to machine-hour) |
Label-free quantification of Phosphoproteome |
Protein extraction- Protein desalting- In-solution digestion(double digestions)- Peptide desalting and quantification-enrichment of phosphorylated peptides-
Peptide desalting -(Peptides fractionation- Peptide desalting)- quantification of peptides- LC MS/MS- Database searching and analysis |
6000 RMB
(Fractionation will increase the fee according to machine-hour) |
TMT quantification of Phosphoproteome |
Protein extraction- Protein desalting- In-solution digestion(double digestions)- Peptide desalting and quantification - TMT labeling and desalting- enrichment of phosphorylated peptides- Peptide desalting and quantification
-( Peptides fractionation- Peptide desalting)- LC MS/MS- Database searching and analysis |
7000 RMB
(Fractionation will increase the fee according to machine-hour) |
TMT quantification of ubiquitproteome |
Protein extraction- Protein desalting- In-solution digestion(double digestions)- Peptide desalting and quantification - TMT labeling and desalting- enrichment of ubiquited peptides- Peptide desalting and quantification
-( Peptides fractionation- Peptide desalting)- LC MS/MS- Database searching and analysis |
10000 RMB (Fractionation will increase the fee according to machine-hour) |
PRM |
First, the PRM uses the quadruple to select the precursor ion with the selection window m/z≤2; the precursor ion is fragmented, and then all product ions are detected by Orbitrap with high resolution and high accuracy. |
2500RMB/protein |
MRM |
First, the MRM uses the quadruple (Q1) to select the precursor ion; then, the precursor ion is fragmented in the collision cell (Q2); finally, Q3 scans targeted product ions with high sensitivity. |
2500RMB/protein |
Orbitrap Fusion,
Q Exactive HF-X |
machine-hour (no more than two hours) |
800RMB/sample |
Q Exactive, Q Exactive plus, QTRAP 6500+ |
machine-hour (no more than two hours) |
600RMB/sample |
Database searching |
Mascot、PD or Maxquant |
100 RMB |
3. Metabolomics |
3.1 GC-MS (A) |
GC-MS |
|
Without any treatment (A1) |
GC-MS- Database searching |
500 RMB |
Complex samples (A2) |
sample purification or derivatization- GC-MS- Database searching |
800 RMB |
Complex samples (A3) |
sample purification and derivatization- GC-MS- Database searching |
1000 RMB |
Absolut quantification (A4) |
GC-MS-data analysis |
600 RMB |
3.2 LC-UV (B) |
|
|
Without any treatment (B1) |
UPLC-UV |
300 RMB |
Complex samples (B2) |
sample purification or derivatization- UPLC-UV-data analysis |
500 RMB |
Complex samples (B3) |
sample purification and derivatization- UPLC-UV-data analysis |
700 RMB |
Absolut quantification (B4) |
UPLC-UV-data analysis |
500 RMB |
3.3 Plant hormone quantification (ABA/JA/SA/IAA/GA/SLs)(C) |
UPLC-MS |
|
1 plant hormone |
plant hormones extraction- UPLC-MS- data analysis |
600 RMB
( If multiple hormones are measured at the same time, the cost will increase according to 200 RMB/ hormone) |
3.4 Plant metabolites quantification (L-Glu/GABA/ O-Acetyl-Serine etal.) (D) |
UPLC-MS |
|
1 plant metabolite |
plant metabolites extraction- UPLC-MS- data analysis |
600 RMB
( If multiple metabolites are measured at the same time, the cost will increase according to 200 RMB/metabolite ) |
|
High Speed Centrifuge |
|
20 RMB/hour |
Super High Speed Centrifuge |
|
50RMB/hour |
Freeze-dryer |
|
20 RMB/hour |
AKTA Protein purification system |
|
300 RMB/ sample |