PSC Frontier Seminar series
Title: The identification and characterization of novel CRISPR effector proteins
Speaker: Prof. Jinsheng Lai, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University.
Time: 10:00 am
Day/Date: July 25th, 2019 (Thursday)
Host: Prof. Jian-Kang Zhu
Venue: PSC Auditorium
CRISPR/Cas systems have been widely repurposed as genome editing or molecular detection tools. Cas9 and Cpf1 are two major CRISPR/Cas effectors recognizing G-rich and T-rich sequences respectively. While several small Cas9 variants being discovered, no small effector recognizing T-rich sequences is available yet. Here we report the identification of additional novel CRISPR/Cas systems: Cas12i and Cas12j. These effectors had both endoribonuclease and single-RNA guided DNA nuclease activity, generating sticky ends and recognizing diverse PAM motifs. Particularly, Cas12i.3 with sufficiently small size (1,045 amino acids) was shown to recognize 5’-TN PAM in vitro. Several Cas12i and Cas12j proteins also have targeted activated trans-ssDNA cleavage activity, thus highly suitable for efficient target sequence detection. Our results expand the diversity of the CRISPR/Cas systems.
The seminar will be delivered in Chinese.