PSC Frontier Seminar series
Title: Development of proteomic strategies to dissect plant signaling networks
Speaker: Dr. HSU/CHUAN CHIH, Department of Plant Biology, Carnegie Institution for Science
Time: 10:00 am
Day/Date: November 8th, 2019 (Friday)
Host: Prof. Pengcheng Wang
Venue: No.1 Meeting room
Kinase-substrate interaction plays a role in the perceiving and responding to many environmental stimuli such as brassinosteroid (BR) pathway in plant cells. Therefore, profiling phosphoproteomic perturbation in response to environmental changes is an efficient way to gain the whole view of plant defense mechanisms. We present a universal sample preparation procedure that facilitates plant phosphoproteomic profiling. We then applied the workflow to study the phosphorylation events involved in tomato cold tolerance mechanisms. To gain insight the kinase-substrate networks involved in environmental stresses, we applied proximity labeling (PL) dependent biotin identification approach in plant tissue to identify transient and stable interactors of BIN2 kinase, a key kinase in BR signaling, in combination with a quantitative kinase-dependent phosphoproteomics study by treatment of plant with a GSK3 kinase inhibitor. Furthermore, we overlap the two datasets to distinguish the BIN2 interacting proteins with the potential direct substrates. Our study demonstrates that TurboID coupled with phosphoproteomics study is a powerful tool for identifying kinase-substrate relationship and dissecting signaling networks in plants.
The seminar will be delivered in English.