PSC Distinguished Seminar series
Title: Toward precision CRISPR DNA fragment editing and predictable 3D genome engineering
Speaker: Prof. Qiang Wu, Distinguished Chair Professor, Shanghai Jiao Tong University
Time: 2:00 pm
Day/Date: April 30th, 2021 (Friday)
Host: Prof. Jian-Kang Zhu
Venue: PSC Auditorium
Ever since gene targeting or specific modification of genome sequences in mice was achieved in the early 1980s, the reverse genetic approach of precise editing of any genomic locus has greatly accelerated biomedical research and biotechnology development. In particular, the recent development of the CRISPR/Cas9 system has greatly expedited genetic dissection of three-dimensional (3D) genomes. CRISPR gene editing results from targeted genome cleavage by ectopic bacterial Cas9 nuclease followed by presumed random ligations via the host double-strand break (DSB) repair machineries. Recent studies revealed, however, that the CRISPR genome editing system is precise and predictable because of cohesive Cas9 cleavage of targeting DNA. I will report our recent progress on the mechanisms of Cas9 gene editing and the principles of CTCF/cohesin-mediated 3D genome folding.
The seminar will be delivered in English.