Phone:(86) 21- 57078272
2014.8: Associate Professor (Junior Group Leader), Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences
2008-2014: Research Associate, Boyce Thompson Institute at Cornell University
2006-2008: Senior Postdoctoral fellow, Dept. of Plant Sciences, ETH-Zurich
2004-2006: Postdoctoral fellow, Dept. of Plant Sciences, ETH-Zurich
2000-2004: PhD., Dept. of Plant Sciences, ETH-Zurich
1998-2000: Research Technician, Dept. of Life Sciences, POSTECH
1998: B.S./M.S., Dongguk University (South Korea), Biology
Ever since the endosymbiotic transfer of plastid genes to the host genome, the development, maintenance and metabolic activity of the plastid have been largely dependent on coordinated gene expression between the host and the endosymbiont, despite the fact that they are physically and biochemically distinct. As the crucial metabolic processes of photosynthesis and photorespiration as well as generation of myriad of molecules including amino acid, secondary metabolite, fatty acid, chemical energy and plant hormones take place in plastid, plastid-to-nucleus retrograde signaling has indispensably evolved. This retrograde communication is essential not only for the biogenesis of plastid but also to integrate a multitude of environmental signals into responses assisting in the fine-tuning of plant development.Hence, a rapid adjustment of plastid homeostasis is possible with the communication, which eventually becomes vital for plants to acclimate under the fluctuating environment.
The primary research themes in the Kim laboratory are perception/responding mechanism of chloroplast to stress, chloroplast-to-nucleus signaling, and chloroplast-mediated stress response including growth inhibition, acclimation and cell death. In addition, we will try to understand the mechanism that underlies chloroplast-mitochondria communication and chloroplast-dependent epigenetic inheritance. In this context, we may eventually understand how chloroplast acts as sensor of multitude of environmental factors and affects plant growth and productivity. The research approach utilizes genetic screen, deep sequencing, proteomics, 540 chloroplast homozygous mutant lines, metabolomics, cell biology and biochemistry in conjunction with plant physiology.
Fig 1. Reactive Oxygen Species (ROS) generation in light-dependent reaction. Is ROS downside or advantage of photosynthesis under adverse environment?
The postdoctoral fellows and PhD candidates will be involved in projects addressing the following questions: 1) revealing perception and response mechanism of chloroplast to stress 2) finding genuine molecules that participate chloroplast-to-nucleus retrograde signaling 3) dissecting the ROS-triggered chloroplast signaling pathway and finding molecular components that relay the signal from chloroplast to nucleus (Fig. 1) 4) understanding acclimation mechanism that is mediated by chloroplasts 5) elucidating subcellular organelles communication with various reporter lines (Fig. 2) 6) building chloroplast phenomics database using chloroplast mutant lines in conjunction with stress responses 7) investigating chloroplast epigenetics.
Fig 2. Integrity of subcellular organelles under adverse environment.Various reporter lines using GFP, CFP, YFP, mCherry and redox-GFP are created to examine the structural integrity of subcellular organelles and their responses to stress.
1. Shengrui Zhang, Klaus Apel* and Chanhong Kim*. Invited to contribute to aTheme Issue for the Journal Philosophical Transactions of the RoyalSociety B: High light signaling and adaptive responses in plants:Singlet oxygen-mediated and EXECUTER-dependent signalling and acclimation of Arabidopsis thaliana exposed to light stress.Philos Trans R SocLond B Biol Sci, 2014 3;369(1640):20130227. *corresponding author
2. Chanhong Kim and Klaus Apel. Singlet oxygen-mediated signaling in plants:A move from flu to wild type reveals an increasing complexity.Photosynthesis Res, 2013 116:455-64
3. Chanhong Kim and Klaus Apel. 1O2-mediated and EXECUTER dependentretrograde plastid-to- nucleus signaling in norflurazon-treated seedlings ofArabidopsis thaliana. Mol Plant, 2013 6:1580-1591
4. Chanhong Kim, Rasa Meskauskiene, Shengrui Zhang, Keun Pyo Lee, AshokM.L, Karolina Blajecka, Magdalena Obazanek-Fojt, Diana Marcela, PazminoMantilla, Ivo Feussner and Klaus Apel. Chloroplasts of Arabidopsis are thesource and a primary target of a plant-specific programmed cell deathsignaling pathway. Plant Cell, 2012 24: 3026-3039
5. Chanhong Kim and Klaus Apel. Arabidopsis light-dependentNADPH:protochlorophyllideoxidoreductase A (PORA) is essential fornormal plant growth and development: An addendum. Plant Molecular Biology, 2012 80: 237-240
6. KláraŠimková, Chanhong Kim, KatarzynaGacekAiswaryaBaruah, ChristopheLaloi and Klaus Apel. The chloroplast division mutant caa33 of Arabidopsisthaliana reveals a crucial impact of chloroplast homeostasis on stressacclimation and retrograde plastid-to-nucleus signaling. Plant Journal, 201269: 701-712
7. Rasa Meskauskiene, Marco Würsch, Christophe Laloi, Pierre-AlexandreVidi,Nuria Sanchez Coll, Felix Kessler, AiswaryaBaruah, Chanhong Kim and KlausApel. A mutation in the mTERF-related plastid protein SOLDAT10 ofArabidopsis activates retrograde signaling and suppresses 1O2-inducedcell death. Plant Journal. 2009 60: 399-410
8. Chanhong Kim*, Keun-Pyo Lee*, AiswaryaBaruah, Mena Nater, Cornelia Göbel,Ivo Feussner and Klaus Apel. 1O2-mediated retrograde signaling during lateembryogenesis pre-determines plastid differentiation in seedlings byrecruiting abscisic acid. PNAS. 2009 106: 9920-9924 * contributed equally tothis work.
9. Chanhong Kim, Rasa Meskauskiene, Klaus Apel and Christophe Laloi. Nosingle way to understand singlet oxygen signaling in plants. EMBO reports.2008, 9: 435-439
10. Keun Pyo Lee*, Chanhong Kim*, Frank Landgraf and Klaus Apel. EXECUTER1-and EXECUTER2-dependent transfer of stress-related signals from theplastid to the nucleus of Arabidopsis thaliana. PNAS. 2007 104:10270-10275* contributed equally to this work.
11. Chanhong Kim, Haemin Ham and Klaus Apel. Multiplicity of different cellandorgans- specific import routes for the NADPHprotochlorophyllide oxidoreductase A and B in plastids of Arabidopsisseedlings.Plant Journal. 2005 42: 329-340
12. Chanhong Kim and Klaus Apel. Substrate-dependent and organ specificchloroplast protein import in planta. Plant Cell. 2004 16: 88-98
13. Chanhong Kim. Substrate-dependent and organ specific chloroplast protein
import in planta. Doctoral Thesis ETH Zurich No. 15409. 2004
14. EnamulHuq, Bassem Al Sady, Matthew Hudson, Chanhong Kim, Klaus Apel,and Peter H. Quail. Phytochrome Interacting Factor1is a Critical bHLH Regulator of Chlorophyll Biosynthesis.Science. 2004 24: 1937-1941
15. Daniela Wagner, DominikaPrzybyla, Roel op den Camp, Chanhong Kim, FrankLandgrf, KeunPyo Lee, Marco Wuersch, Christophe Laloi, Mena Nater,Eva Hideg, and Klaus Apel. The Genetic Basis of Singlet Oxygen inducedStress Responses of Arabidopsis thaliana.Science. 2004 12: 1183-1185
16. David Goslings, Rasa Meskauskiene, Chanhong Kim, Keun Pyo Lee, Mena Nater and Klaus Apel. Concurret interactions of heme and FLU with GlutRNAreductase, the target of metabolic feedback inhibition oftetrapyrrole biosynthesis in higher plants. Plant Journal. 2004 40: 957-967
17. Keun Pyo Lee*, Chanhong Kim*, Dae Won Lee and Klaus Apel.TIGRINA d, required for regulating the biosynthesis of tetrapyrroles in barley, is an ortholog of the FLU gene of Arabidopsis thaliana.FEBS Lett. 2003 553: 119-124 * contributed equally to this work.
18. Roel G. L. op den Camp, DominikaPrzybyla, Christian Ochsenbein, Christophe Laloi, Chanhong Kim,Antoine Danon, Daniela Wagner, Eva Hideg, Cornelia Gibel,Ivo Feussne, Mena Nater and Klaus Apel.Rapid induction of distinct stress responses after the release of singlet oxygen in Arabidopsis.Plant Cell. 2003 15: 2320-2332
19. Seonghoe Jang, Byongho Lee, Chanhong Kim, Soo-Jin Kim, JieunYim, JongJin Han, Shinyoung Lee, Seong-Ryong Kim and Gynheung An.The OsFOR1 gene encodes a polygalacturonaseinhibiting protein (PGIP) that regulates floral organ number in rice.Plant Molecular Biology. 2003 53: 357-372
20. Dae-Won Lee, Chanhong Kim and Dong-Ung Lee.Effect of cultural conditions on the biosynthesis of gagaminine, a potent antioxidant from the roots of Cynanchumwilfordii.Biological & Pharmaceutical Bulletin. 2001 24: 1451-1453
21. Chanhong Kim, Dong-HoonJeong and Gynheung An.Molecular cloning and characterization of OsLRK1 encoding a putative receptor-like protein kinase from Oryza sativa. Plant Science. 2000 152:17-26
22. Jong-Seong Jeon, Seonghoe Jang, Sichul Lee, Jongmin Nam, Chanhong Kim,Sang-Hee Lee, Yong-Yoon Chung, Seong-Ryong Kim, YeonHee Lee, YongGu Cho and Gynheung An. Leafy hull sterile1 is a homeotic mutation in a Rice MADS box gene affecting rice flower development.Plant Cell. 2000 12: 871-884.
23. Jong-Seong Jeon, Sichul Lee, Ki-Hong Jung, Sung-Hoon Jun, DongHoonJeong, Jinwon Lee, Chanhong Kim, Seonghoe Jang, ShinyoungLee, Kiyoung Yang, Jongmin Nam, Kyungsook An, Min-Jung Han, Ryo-Jin Sung, Hyun-Sook Choi, Jung Hwa Yu, Jung-Hwan Choi, Se-Yu Cho, Sang-Su Cha, Shi-In Kim and Gynheung An. T-DNA insertional mutagenesis forfunctional genomics in rice. Plant Journal. 2000 22: 561-570
24. Jong-Seong Jeon, Sichul Lee, Ki-Hong Jung, Sung Hoon Jun, Chanhong Kimand Gynheung An. Tissue-preferential expression of a Rice α tubulin Gene,OsTubA1, mediated by the first intron. Plant Physiol. 2000 123: 1005 -1001