学术活动
导师介绍

段成国
研究员,博士生导师


Email: cgduan@sibs.ac.cn

个人简介:
1997.09-2001.07:山东农业大学,学士学位

2001.09-2004.07:山东农业大学,硕士学位

2004.09-2008.07:中国科学院微生物研究所,博士学位

2008.07-2011.10:中国科学院微生物研究所,助理研究员

2011.10-2016.10:美国普渡大学,博士后

2016.11-至今:中国科学院上海植物逆境生物学研究中心,研究员

研究方向:
植物逆境胁迫(生物与非生物逆境)的表观遗传响应机制

研究工作:
植物在生长发育过程中会面对各种各样的逆境胁迫,包括旱涝、极端温度、高盐等非生物逆境胁迫以及病虫害等生物逆境胁迫。表观遗传途径作为真核生物中一种非常保守的精细调控通路具有反应迅速、可逆等特点,因此在植物与环境因子的互作中发挥重要作用。本研究组以模式植物拟南芥及主要农作物为研究材料,以遗传学、分子生物学、生物化学及生物信息学为主要研究手段,剖析植物逆境胁迫调控的分子机制及其与表观遗传途径的互作。具体研究内容包括:





1. 表观遗传途径介导的基因表达与RNA加工机制及其对植物生长发育与抗逆的调控。
2. 植物非生物逆境胁迫响应过程中表观遗传途径(包括DNA甲基化、非编码RNA与组蛋白修饰等内容)的调控机制及其应用;
3. 植物-病原(生物逆境胁迫)互作的表观遗传调控机制及抗性育种;

获奖情况:
“Excellent Scholar Award” of the XIX International Botanical Congress

代表性论文:
1. Duan C.G.#*, Wang X.#, Zhang L., Xiong X., Zhang Z., Tang K., Pan L., Hsu C.C., Xu H., Tao A.W., Zhang H., Zhu J.K.* (2017) A protein complex regulates RNA processing of intronic heterochromatin-containing genes in Arabidopsis. PNAS (in press) (* Co-corresponding author)

2. Duan C.G.#, Wang X.#, Xie S., Pan L., Miki D., Tang K., Hsu C.C., Lei M., Zhong Y., Hou Y.J., Wang Z., Zhang Z., Mangrauthia S.K., Xu H., Zhang H., Dilkes B., Tao W.A., Zhu J.K.* (2017) A pair of transposon-derived proteins function in a histione acetyltransferase complex for active DNA demethylation. Cell Research 27(2): 226-240

3. Duan C.G.*, Zhu J.K. (2017) Dissecting the Subnuclear Localization Patterns of Argonaute Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants. Methods in Molecular Biology 1640: 129-135. (* Corresponding author)

4. Tang K.*, Duan C.G., Zhang H., Zhu J.K. (2017) Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants. Methods in Molecular Biology1640: 219-225

5. Zhao J.H., Fang Y.Y., Duan C.G., Fang R.X., Ding S.W., Guo H.S.* (2016) Genome-wide identification of endogenous RNA-directed DNA methylation loci associated with abundant 21-nucleotide siRNAs in Arabidopsis. Scientific Reports 6: 36247

6. Fang Y.Y.#, Zhao J.H.#, Liu S.W., Wang S., Duan C.G.*, Guo H.S.* (2016) CMV2b-AGO interaction is required for the suppression of RDR-dependent antiviral silencing in Arabidopsis. Frontiers in Microbiology 7: 1329 (* Co-corresponding author)

7. Duan C.G.#, Wang X.#, Tang K., Zhang H., Mangrauthia S.K., Lei M., Hsu C.C., Hou Y.J., Wang C., Li Y., Tao W.A. and Zhu J.K.* (2015) MET18 Connects the Cytosolic Iron-Sulfur Cluster Assembly Pathway to Active DNA Demethylation in Arabidopsis. PLoS Genetics 11(10): e1005559

8. Li Y.#, Duan C.G.#, Zhu X., Qian W. and Zhu J.K.* (2015) A DNA ligase required for active DNA demethylation and genomic imprinting in Arabidopsis. Cell Research 25: 757-760 (# Co-first author)

9. Duan C.G.#, Zhang H.#, Tang K.#, Zhu X, Qian W, Hou YJ, Wang B, Lang Z, Zhao Y, Wang X, Wang P, Zhou J, Liang G, Liu N, Wang C, Zhu J.K.*. (2014) Specific but Interdependent Functions for Arabidopsis AGO4 and AGO6 in RNA-directed DNA Methylation. EMBO Journal 34(5): 581-592

10. Lang Z, Lei M, Wang X, Tang K, Miki D, Zhang H, Mangrauthia SK, Liu W, Nie W, Ma G, Yan J, Duan C.G., Hsu CC, Wang C, Tao WA, Gong Z, Zhu J.K. (2015) The Methyl-CpG-Binding Protein MBD7 Facilitates Active DNA Demethylation to Limit DNA Hyper-Methylation and Transcriptional Gene Silencing. Molecular Cell 57: 971-983

11. Zhang H., Tang K., Qian W., Duan C.G., Wang B., Zhang H., Wang P., Zhu X., Lang Z., Yang Y., Zhu J.K.* (2014) An Rrp6-like protein positively regulates noncoding RNA levels and DNA methylation in Arabidopsis. Molecular Cell 54: 418-430

12. Zhao Y, Xing L, Wang X, Hou Y.J., Gao J, Wang P, Duan C.G., Zhu X, Zhu J.K. (2014) The ABA receptor PYL8 promotes lateral root growth by enhancing MYB77-dependent transcription of auxin-responsive genes. Science signaling 7: ra53

13. Lei, M., La, H., Lu, K., Wang, P., Miki, D., Ren, Z., Duan, C.G., Wang, X., Tang, K., Zeng, L., Yang L., Zhang H., Nie W., Liu P., Zhou J., Liu R., Zhong Y., Liu D and Zhu J.K.* (2014). Arabidopsis EDM2 promotes IBM1 distal polyadenylation and regulates genome DNA methylation patterns. PNAS 111, 527-532

14. Wang X.#, Duan C.G.#, Tang K., Wang B., Zhang H., Lei M., Lu K., Mangrauthia S.K., Wang P., Zhu G., Zhao Y. and Zhu J.K.* (2013). RNA-binding protein regulates plant DNA methylation by controlling Mrna processing at the intronic heterochromatin- containing gene IBM1. PNAS 110(38): 15467-15472 (# Co-first author)

15. Feng L.#, Duan C.G.# and Guo H.S.* (2013). Inhibition of in vivo Slicer activity of Argonaute protein 1 by the viral 2b protein independent of its dsRNA-binding function. Molecular Plant Pathology 14, 617-622. (# Co-first author)

16. Zhao, Y., Chan, Z., Xing, L., Liu, X., Hou, Y.J., Chinnusamy, V., Wang, P., Duan, C.G. and Zhu, J.K.* (2013). The unique mode of action of a divergent member of the ABA-receptor protein family in ABA and stress signaling. Cell Research 23, 1380-1395

17. Zhang H., Wang B., Duan C.G., and Zhu J.K.* (2013). Chemical probes in plant epigenetics studies. Plant Signaling & Behavior 8.

18. Duan C.G.#, Fang Y.Y.#, Zhou B.J., Zhao J.H., Hou W.N., Zhu H., Ding S.W., and Guo H.S. (2012). Suppression of Arabidopsis ARGONAUTE1-mediated slicing, transgene-induced RNA silencing, and DNA methylation by distinct domains of the Cucumber mosaic virus 2b protein. Plant Cell 24, 259-274.

19. Duan C.G., Wang C.H., and Guo H.S.* (2012). Application of RNA silencing to plant disease resistance. Silence 3: 5.

20. Zhu H., Duan C.G., Hou W.N., Du Q.S., Lv D.Q., Fang R.X., and Guo H.S.* (2011). Satellite RNA-derived small interfering RNA satsiR-12 targeting the 3' untranslated region of Cucumber mosaic virus triggers viral RNAs for degradation. Journal of Virology 85, 13384-13397.

21. Hou W.N.#, Duan C.G.#, Fang R.X., Zhou X.Y., and Guo H.S.* (2011). Satellite RNA reduces expression of the 2b suppressor protein resulting in the attenuation of symptoms caused by Cucumber mosaic virus infection. Molecular Plant Pathology 12, 595-605. (# Co-first author)

22. Ying X.B., Dong L., Zhu H., Duan C.G., Du Q.S., Lv D.Q., Fang Y.Y., Garcia J.A., Fang R.X., and Guo H.S.* (2010). RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana. Plant Cell 22, 1358-1372

23. Zheng N., Xia R., Yang C., Yin B., Li Y., Duan C.G., Liang L., Guo H.S., and Xie Q.* (2009). Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice. Vaccine 27, 5001-5007

24. Duan C.G., Wang C.H., Fang R.X., and Guo H.S.* (2008). Artificial MicroRNAs highly accessible to targets confer efficient virus resistance in plants. Journal of Virology 82, 11084-11095.

25. Duan C.G., Wang C.H. & Guo H.S.* (2008). Delayed resistance to Cucumber Mosaic Virus mediated by 3’UTR-derived hairpin RNA. Chinese Science Bulletin 53 (21): 3301-3310

26. Du Q.S.#, Duan C.G.#, Zhang Z.H.#, Fang Y.Y., Fang R.X., Xie Q., and Guo H.S.* (2007). DCL4 targets Cucumber mosaic virus satellite RNA at novel secondary structures. Journal of Virology 81, 9142-9151. (# Co-first author)

27. Duan C.G., Wang C.H. & Guo H.S.* (2006). Regulation of microRNA on plant development and viral infection. Chinese Science Bulletin 51(3): 269-278.