学术活动
导师介绍

黄朝锋
研究员,博士生导师


Email: cfhuang@sibs.ac.cn
Phone:54924320

个人简介:
1996—2000年 华南农业大学,学士学位
2000—2003年 华南农业大学,硕士学位
2003—2004年 中科院上海生科院植物生理生态研究所,研究助理
2004—2006年 日本香川大学,硕士学位
2006—2009年 日本爱媛大学,博士学位
2009—2010年 日本冈山大学,特别契约助理教授
2010—2012年 美国加州大河滨分校、普渡大学,博士后
2012—2016年 南京农业大学,教授
2016—至今 中科院上海植物逆境生物学研究中心,研究员

研究方向:
植物金属逆境生物学

研究工作:
(1)植物耐铝毒机制的研究
酸性土壤占世界耕地的30%以上,并且随着过度频繁地耕作和氮肥的过度使用土壤有进一步酸化的趋势。铝在地壳中是最丰富的金属元素,约占7%,但只有在酸性土壤中三价铝离子(Al3+)才被溶解出并毒害植物根系的生长,进而影响作物的生长发育,最终导致作物减产。因此,铝毒被认为是仅次于干旱胁迫的第二大非生物逆境。为了应对酸性土壤的铝毒害,植物进化了包括分泌有机酸、修饰细胞壁、隔离铝到液泡中等一系列耐铝毒机制。虽然耐铝毒机制的研究已经取得较大进展,但是仍有很多未知的机制等待我们去解析,包括铝毒受体,铝毒在细胞内的信号转导过程,耐铝毒转录因子STOP1/ART1的转录后调控机制等等。我们以模式植物拟南芥和模式作物水稻为研究对象,采用正向遗传筛选策略鉴定耐铝毒新基因。目前我们已经筛选获得一系列铝毒敏感或铝抗突变体,并克隆了若干个耐铝毒新基因。接下来我们将采用遗传、基因组学、细胞生物学、生理生化等各种技术手段研究目的基因的功能,揭示植物耐铝毒新机制。

(2)植物锰利用机制的研究
锰参与植物光合、酶氧化还原、脂类合成等过程,是植物必需的矿质营养元素。在碱性土壤中,由于锰的生物有效性降低,植物容易出现缺锰症状。虽然目前关于锰的吸收和转运机制方面已经取得较大进展,但是关于锰在细胞内的分配与利用机制还了解甚少,尤其是锰在叶绿体中的转运和利用机制。我们筛选获得一系列影响锰在叶绿体中利用的突变体,计划通过克隆和功能解析目的基因来阐明锰在叶绿体中的运输和利用机制。

研究生情况:
指导博士研究生7名,硕士研究生12名

代表性论文:

23. Zhang Y#, Zhang J#, Guo JL#, Zhou FL, Singh S, Xu X, Xie Q, Yang ZB and Huang CF* (2018) F-box protein RAE1 regulates the stability of the aluminum-resistance transcription factor STOP1 in Arabidopsis. Proc Natl Acad Sci USA doi/10.1073/pnas.1814426116.

22. Zhu HF#, Xie WX#, Xu DC#, Miki D, Tang K, Huang CF* and Zhu JK* (2018) DNA demethylase ROS1 negatively regulates the imprinting of DOGL4 and seed dormancy in Arabidopsis thaliana. Proc Natl Acad Sci USA 115: E9962-E9970.

21. Yang CH#, Zhang Y# and Huang CF* (2018) Reduction in cadmium accumulation in japonica rice grains by CRISPR/Cas9-mediated editing of OsNRAMP5. Journal of Integrative Agriculture 17:60345-7

20. Zhang Y#, Guo JL#, Chen M, Li L, Wang LH and Huang CF* (2018) The cell cycle checkpoint regulator ATR is required for internal aluminum toxicity-mediated root growth inhibition in Arabidopsis. Frontiers in Plant Science 9:118

19. Gao HL, Xie WX, Yang CH, Xu JY, Li JJ, Wang H, Chen X and Huang CF* (2018) NRAMP2, a trans-Golgi network localized manganese transporter, is required for Arabidopsis root growth under manganese deficiency. New Phytologist 217:179-193.

18. Liu S, Gao HL, Wu XY, Fang Q, Chen L, Zhao FJ and Huang CF* (2016) Isolation and characterization of an aluminum-resistant mutant in rice. Rice 9:60.

17. Yan JL, Wang PT, Wang P, Yang M, Lian XM, Tang Z, Huang CF, Salt DE and Zhao FJ (2016) A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars. Plant Cell & Environment 39:1941–1954.

16. Yang Y, Liu Y, Huang CF, Silva J and Zhao FJ (2016) Aluminium alleviates fluoride toxicity in tea (Camellia sinensis). Plant Soil 402:179-190.

15. Zhu HF, Wang H, Zhu YF, Zou JW, Zhao FJ and Huang CF* (2015) Genome-wide transcriptomic and phylogenetic analyses reveal distinct aluminum-tolerance mechanisms in the aluminum-accumulating species buckwheat (Fagopyrum tataricum). BMC Plant Biology 15:16.

14. Yang M, Zhang YY, Zhang LJ, Hu JT, Zhang X, Lu K, Dong HX, Wang DJ, Zhao FJ, Huang CF and Lian XM (2014) OsNRAMP5 contributes to manganese translocation and distribution in rice shoots. Journal of Experimental Botany 65: 4849–4861.

13. Huang CF* and Zhu JK* (2014) RNA splicing factors and RNA-directed DNA methylation. Biology 3:243-254.

12. Huang CF, Miki D, Tang K, Zhou HR, Zheng Z, Chen W, Ma ZY, Yang L, Zhang H, Liu R, He XJ, Zhu JK (2013) A pre-mRNA-splicing factor is required for RNA-directed DNA methylation in Arabidopsis. PLOS Genetics 9: e1003779.

11. Dou K#, Huang CF#, Ma ZY, Zhang CJ, Zhou JX, Huang HW, Cai T, Tang K, Zhu JK and He XJ (2013) The PRP6-like splicing factor STA1 is involved in RNA-directed DNA methylation by facilitating the production of Pol V-dependent scaffold RNAs. Nucleic Acids Research 41: 8489-8502.

10. Tsutsui T, Yamaji N, Huang CF, Motoyama R, Nagamura Y, Ma JF (2012) Comparative genome-wide transcriptional analysis of Al-responsive genes reveals novel Al tolerance mechanisms in rice. PLOS ONE 7: e48197.

9. Huang CF, Yamaji N, Chen ZC and Ma JF (2012) A tonoplast-localized half-size ABC transporter is required for internal detoxification of Al in rice. Plant Journal 69: 857-867.

8. Huang CF, Yamaji N, Ono K and Ma JF (2012) A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots. Plant Journal 69: 565–741 (Cover story).

7. Ueno D, Yamaji N, Kono I, Huang CF, Ando T, Yano M and Ma JF (2010) Gene limiting cadmium accumulation in rice. Proc Natl Acad Sci USA 107: 16500-16505.

6. Huang CF, Yamaji N, and Ma JF (2010) Knockout of a bacterial-type ATP-binding cassette transporter gene, AtSTAR1, results in increased aluminum sensitivity in Arabidopsis. Plant Physiology 153: 1669-1677.

5. Yamaji N#, Huang CF#, Nagao S, Yano M, Sato Y, Nagamura Y, and Ma JF (2009) A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. Plant Cell 21: 3339-3349.

4. Huang CF, Yamaji N, Nishimura M, Tajima S, and Ma JF (2009) A rice mutant sensitive to Al toxicity is defective in the specification of root outer cell layers. Plant and Cell Physiology 50: 976-985.

3. Wang LP, Yin HF, Qian Q, Yang J, Huang CF, Hu XH, and Luo D (2009) NECK LEAF 1, a GATA type transcription factor, modulates organogenesis by regulating the expression of multiple regulatory genes during reproductive development in rice. Cell Research 19: 598-611.

2. Huang CF#, Yamaji N#, Mitani N, Yano M, Nagamura Y, and Ma JF (2009) A bacterial-type ABC transporter is involved in aluminum tolerance in rice. Plant Cell 21: 655-667.

1. Ma JF, Nagao S, Huang CF, and Nishimura M (2005) Isolation and characterization of a rice mutant hypersensitive to Al. Plant and Cell Physiology 46: 1054-1061.